Email this article Pengaruh Ekstrak Buah Pare (Momordica charantia L.) Terhadap Struktur Mikroskopis Hati Mencit (Mus musculus)
Abstract
Momordica charantia L. (bitter melon) contains various bioactive compounds that may affect internal organs, including the liver. This study aimed to examine the effect of bitter melon fruit extract on the microscopic structure of the liver in mice (Mus musculus). The research was conducted experimentally using a Completely Randomized Design (CRD) without factorial treatment, consisting of five groups: untreated control, aquadest control, and treatment groups receiving bitter melon extract at doses of 750 mg/kg BW, 1500 mg/kg BW, and 2250 mg/kg BW, with five replications each. The extract was administered orally using a gavage needle at a volume of 0.1 mL/10 g BW daily for 40 days. The results showed that the extract did not significantly affect body weight or liver weight. Microscopically, the 750 mg/kg BW and 1500 mg/kg BW doses did not alter liver histology compared to the control, while the 2250 mg/kg BW dose caused histological damage in the form of hepatocyte steatosis. Macroscopically, the liver in the highest dose group appeared paler than in the control group, although the overall shape remained normal in all groups. It can be concluded that high doses of bitter melon extract may induce microscopic liver changes, whereas lower doses do not exhibit hepatotoxic effects.
Keywords
Full Text:
PDFReferences
Abdillah, S., Inayah, B., Febrianti, A. B., & Nafisa, S. (2020). Acute and Subchronic Toxicity of Momordica charantia L Fruits Ethanolic Extract in Liver and Kidney. Systematic Reviews in Pharmacy, 11(12).
Amaliyah, S. (2023). Potensi Daun Kelor sebagai Herbal untuk Penyakit Hati Stadium Lanjut. Universitas Brawijaya Press.
AZIZ, J., Qonitah, F., & Ariastuti, R. (2021). Uji Kandungan Flavonoid Total Fraksi Ekstrak Etanol 70% Daging Buah dan Biji Pare (Momordica charantia L.) (Doctoral dissertation, Universitas Sahid Surakarta).
Azizah, Z., & Wati, S. W. (2018). Skrining fitokimia dan penetapan kadar flavonoid total ekstrak etanol daun Pare (Momordica charantia L.). Jurnal Farmasi Higea, 10(2), 163-172.
Jeon, A., Cheon, W., Yoon, J., Kim, D. J., & Kim, Y. (2020). Antioxidant and Hepatoprotective Activities of Bitter Melon (Momordica charantia Linn.) Leaves against Oxidative Stress. Journal of the Korean Society of Food Culture, 35(6), 597-604.
Lestari, T. S., & Hamzah, B. (2022). Analisis kadar senyawa flavonoid ekstrak etanol buah pare (Momordica charantia l.). Media Eksakta, 18(2), 96-101.
Lubis, W. R., & Syukriah, S. (2025). The Effect of Giving Ethanol Extract of Palm Fruit (Arenga pinnata Merr.) on Morphology and Liver Function of White Rats (Rattus norvegicus L.) Induced by Carbon Tetrachloroide. Jurnal Biologi Tropis, 25(2), 1269-1274.
Mobasheri, L., Ahadi, M., Namdar, A. B., Alavi, M. S., Bemidinezhad, A., Farahi, S. M. M., ... & Ghorbani, A. (2023). Pathophysiology of diabetic hepatopathy and molecular mechanisms underlying the hepatoprotective effects of phytochemicals. Biomedicine & Pharmacotherapy, 167, 115502.
Muhtadi, M., & Pangestuti, Y. S. (2019, October). Aktivitas Antidiabetes dari Kombinasi Serbuk Ikan Gabus (Channa striata) dan Ekstrak Etanol Buah Pare (Momordica charantia L) terhadap Tikus Wistar Jantan yang Diinduksi Aloksan. In Prosiding University Research Colloquium (pp. 40-47).
Mukminin, A. U. (2024). Studi penambatan molekul (molecular docking) senyawa buah pare (Momordica charantia L.) golongan flavonoid sebagai antidiabetes pada reseptor ppar (Doctoral dissertation, Universitas Islam Negeri Maulana Malik Ibrahim).
Munaeni, W., Mainassy, M. C., Puspitasari, D., Susanti, L., Endriyatno, N. C., Yuniastuti, A., ... & Hendra, G. A. (2022). Perkembangan Dan Manfaat Obat Herbal Sebagai Fitoterapi. Tohar Media.
Mundiri, N. A., Maulida, M., Tejasari, M., Furqaani, A. R., & Ekowati, R. R. (2019). Pengaruh fraksi air buah lemon terhadap gambaran morfologi jaringan hati mencit tua yang diberi pakan tinggi lemak protective effect of water fraction of lemon on high-fat diet-induced liver injury in old mice. J Integr Kesehat Sains, 1(22), 49-53.
Offor, U., Naidu, E. C., Ogedengbe, O. O., Aniekan, P. I., & Azu, O. O. (2020). Momordica charantia mitigates hepatic injury following adjuvant treatment with antiretroviral drugs in diabetic animal models. Toxicological Research, 36, 37-44.
Oktarian, A., Budiman, H., & Aliza, D. (2017). Histopatologi Hati Tikus Putih (Rattus novergicus) Yang Diinjeksi Formalin (Histopathology of Rat (Rattus novergicus) Liver Induced by Formaldehide). Jurnal Ilmiah Mahasiswa Veteriner, 1(3), 316-323.
Puspitasari, V., & Choerunisa, N. (2021). Kajian sistematik: efek anti diabetes buah pare (Momordica charantia Linn.) terhadap kadar glukosa darah pada tikus yang diinduksi aloksan. Generics: Journal of Research in Pharmacy, 1(2), 18-27.
Ramadhan, S., Ramadhan, A., Laenggeng, A. H., & Kundera, I. N. (2021). Pengaruh Kombinasi Ekstrak Daun Pare (Momordica charantia L.) dan Kunyit (Curcuma longa) Terhadap Kadar Kreatinin Pada Tikus (Rattus norvegicus) yang Diinduksi CCl4. Journal of Biology Science and Education, 9(2), 780-785.
Ramos, L. F., Silva, C. M., Pansa, C. C., & Moraes, K. C. (2021). Non-alcoholic fatty liver disease: molecular and cellular interplays of the lipid metabolism in a steatotic liver. Expert Review of Gastroenterology & Hepatology, 15(1), 25-40.
Saino, S., Yuniatun, S. R. A., & Susanto, A. (2022). Implementasi Jus Buah Pare pada Perawatan Pasien Diabetes Mellitus dengan Masalah Ketidakstabilan Kadar Gula Darah di Ruang Kenari Atas RSUD Ajibarang. Jurnal Inovasi Penelitian, 3(4), 5717-5724.
Sikteubun, C. A., Hasan, D., & Abdillah, S. (2019). Efikasi Dan Keamanan Kapsul Pare (Momordica charantia L)-Primakuin Dan Dihidroartemisin Piperaquin-Primakuin Pada Pasien Malaria Vivax Di RSUD Manokwari Tahun 2019. J Ilmu Kedokteran, 6(3).
Sriwijayanti, S., Situmeang, B., Yulianti, N., Susvira, D., & Widiyanto, H. (2024). Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Biji Buah Pare (Momordica charantia L.). Jurnal Beta Kimia, 4(1), 77-84.
Taupik, M., Djuwarno, E. N., & Mustapa, M. A. (2021). Kajian fitokimia dan identifikasi senyawa metaboli sekunder daun pare (Momordica charantia L.). Al-Kimia, 9(2), 170-181.
Triyudha, G., Febriani, H., & Hutasuhut, M. A. (2025). Pengaruh Pemberian Ekstrak Etanol Buah Aren (Arenga pinnata Merr.) Terhadap Profil Darah Tikus Putih (Rattus norvegicus L.) Yang Diinduksi Karbon TetraKlorida (CCl4). Jurnal Biogenerasi, 10(2), 1182-1189.
Wahyuni, F. S., Putri, I. N., & Arisanti, D. (2017). uji toksisitas subkronis fraksi etil asetat kulit buah asam kandis (Garcinia cowa Roxb.) terhadap fungsi hati dan ginjal mencit putih betina. Jurnal Sains Farmasi & Klinis, 3(2), 202-212.
Yusuf, A. L., Nugraha, D., Wahlanto, P., Indriastuti, M., Ismail, R., & Himah, F. A. (2022). Formulasi Dan Evaluasi Sediaan Gel Ekstrak Buah Pare (Momordica charantia L.) Dengan Variasi Konsentrasi Carbopol 940. Pharmacy Genius, 1(1), 50-61.
Zheng, J., Shang, M., Dai, G., Dong, J., Wang, Y., & Duan, B. (2024). Bioactive polysaccharides from Momordica charantia as functional ingredients: A review of their extraction, bioactivities, structural-activity relationships, and application prospects. Critical Reviews in Food Science and Nutrition, 64(32), 12103-12126.
DOI: https://doi.org/10.31289/jibioma.v7i1.6118
Refbacks
- There are currently no refbacks.
Copyright (c) 2025 Jurnal Ilmiah Biologi UMA (JIBIOMA)
This work is licensed under a Creative Commons Attribution 4.0 International License