Pengukuran Aktivitas Nitrat Reduktase (ANR) Pada Tanaman Poaceae Secara In vivo

Devi Bunga Pagalla, Magfirahtul Jannah

Abstract


Corn, rice, and sugarcane are included in the Poaceae family which have high economic value as staple foods. Nitrate reductase plays a role in reducing nitrate to ammonia. The nitrate reductase activity testing can help biotechnology studies, in this case, fertilizer production or organic waste treatment, because the nitrate reductase enzyme can be used to convert nitrate into more useful nitrogen compounds. The purpose of this study was to analyze the activity of nitrate reductase (ANR) from the leaves of the Poaceae family, namely corn, rice, and sugarcane. In vivo ANR measurements were carried out using the spectrophotometric method with two types of spectrophotometers, namely the Vis/UV Spectrophotometer, Genesys10UV, and Visible Spectrophotometer, with a wavelength of 540 nm. This study concludes that rice had the highest ANR of 0.1503 µmol/gram leaf weight/hour of incubation. The increase in nitrate reductase activity indicates that the energy for nitrate reduction also increases and provides a large capacity for synthesizing amino acids, proteins, or total N assimilation.


Keywords


Zea mays; Oryza sativa; Saccharum officinarum; ANR; In vivo; Spectrophotometric

Full Text:

PDF

References


Adhikari, K., Bhandari, S., Aryal, K., Mahato, M., & Shrestha, J. (2021). Effect of different levels of nitrogen on growth and yield of hybrid maize (Zea mays L.) varieties. Journal of Agriculture and Natural Resources, 4(2), 48-62.

Brahmanta, A. (2021). Potensi Terapi Hiperbarik Oksigen Dalam Ortodonti: Percepatan Pergerakan Gigi. Airlangga University Press.

Chamizo-Ampudia, A., Sanz-Luque, E., Llamas, A., Galvan, A., & Fernandez, E. (2017). Nitrate reductase regulates plant nitric oxide homeostasis. Trends in Plant Science, 22(2), 163-174.

Ende, S., Salawati, S., Kadekoh, I., Fathurrahman, F., Darman, S., & Lukman, L. (2022). Aktivitas Nitrat Reduktase (ANR) Tanaman Jagung pada Pola Tumpangsari yang Diberi Serasah Jagung-Kedelai serta Biochar di Lahan Suboptimal Sidondo Sulawesi Tengah. Jurnal Ilmu Pertanian Indonesia, 27(4), 528-535.

Fu, Y. F., Zhang, Z. W., & Yuan, S. (2018). Putative connections between nitrate reductase S-nitrosylation and NO synthesis under pathogen attacks and abiotic stresses. Frontiers in Plant Science, 9, 474.

Koryati, T., Purba, D. W., Surjaningsih, D. R., Herawati, J., Sagala, D., Purba, S. R., ... & Aldya, R. F. (2021). Fisiologi tumbuhan. Yayasan Kita Menulis.

Mastur, M., Syafaruddin, S., & Syakir, M. (2015). Peran dan pengelolaan hara nitrogen pada tanaman tebu untuk peningkatan produktivitas tebu. Perspektif: Review Penelitian Tanaman Industri, 14(2), 73-86.

Murtiyaningsih, H., & Hazmi, M. (2017). Isolasi dan uji aktivitas enzim selulase pada bakteri selulolitik asal tanah sampah. Agritrop: Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 15(2). Kolbert, Z., Bartha, B., and Erdei, L. (2008). Exogenous auxin-induced NO synthesis is nitrate reductase-associated in Arabidopsis thaliana root primordia. J. Plant Physiol. 165, 967–975. doi: 10.1016/j.jplph.2007.0 7.019.

Peni, D. K., & Solichatun, A. E. (2004). Pertumbuhan, Kadar Klorofil-Karotenoid, Saponin, Aktivitas Nitrat reduktase Anting-anting (Acalypha indica L.) pada Konsentrasi Asam Giberelat (GA3) yang Berbeda. Biofarmasi, 2(1), 1-8.

Rockel, P., Strube, F., Rockel, A., Wildt, J., & Kaiser, W. M. (2002). Regulation of nitric oxide (NO) production by plant nitrate reductase in vivo and in vitro. Journal of experimental botany, 53(366), 103-110.

Sadikin, M. (2019). Biokomia Enzim. Pustaka Poltekkes Padang. Padang.

Sari, R., & Prayudyaningsih, R. (2015). Rhizobium: pemanfaatannya sebagai bakteri penambat nitrogen. Buletin Eboni, 12(1), 51-64.

Simpson, M. G. (2019). Plant systematics. Academic press.

Urban, A., Rogowski, P., Wasilewska-Dębowska, W., & Romanowska, E. (2021). Understanding maize response to nitrogen limitation in different light conditions for the improvement of photosynthesis. Plants, 10(9), 1932.

Xu, Z., Chen, Y., Gartia, M. R., Jiang, J., & Liu, G. L. (2011). Surface plasmon enhanced broadband spectrophotometry on black silver substrates. Applied Physics Letters, 98(24), 241904.

Zhang, X. C., & Shanguan, Z. P. (2007). Nitrogen Regulatory Metabolism in leaf membrane superoxidation on winter wheat with diferent drought resistant abilities. Plant Nutrition. Fertility Science, 13(1), 106-112.




DOI: https://doi.org/10.31289/jibioma.v5i1.1681

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Jurnal Ilmiah Biologi UMA (JIBIOMA)

Fakultas Biologi
Universitas Medan Area
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License